Hi Lanzo -
As LikesFish said - no - RO water cannot cause this. Even tough you don't have a DSB, it looks like you sand-bed in the DT is at least 4-5cm deep. So, it could be the start of denitrification. BUT, as I said - I see a lot of green algae in the top surface layer of the substrate - so, as LikesFish has said - if this happens in the day - then it could definitely be oxygen production.... and perhaps carbon dioxide production in the night-time...
As LikesFish said - no - RO water cannot cause this. Even tough you don't have a DSB, it looks like you sand-bed in the DT is at least 4-5cm deep. So, it could be the start of denitrification. BUT, as I said - I see a lot of green algae in the top surface layer of the substrate - so, as LikesFish has said - if this happens in the day - then it could definitely be oxygen production.... and perhaps carbon dioxide production in the night-time...