Can be done. My substrate is sand collected from under the low water mark. Depending on how much sand you would need. Just take note, wet sand is very heavy. You will have die-off. I will not add sand just collected into a running system, especially not after a couple of hours drive. Did my tank when I started it up, had enough time to let it cycle before moving the old system over.
Here it was cycling. The sump was already connected to the old system, so I run the display with half filled for some time with a few small powerheads and skimmer inside the display.