Proper Acclimatisation

Discussion in 'Fish Articles' started by Mekaeel, 20 May 2008.

  1. Mekaeel

    Mekaeel Moderator

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    Hey guys,figured this article might help our newbies.
    ACCLIMATION METHODS

    Proper acclimation actually helps reduce the stress animals face after relocation. In fact, the acclimation procedure itself is fairly simple—although it does vary slightly for different species of animals. Sensitive fish, corals and invertebrates, such as snails, crabs and shrimp take longer to acclimate than hardier fish, corals and inverts.


    Temperature

    The temperature inside the package or bag your livestock arrives in is undoubtedly different than the temperature inside your aquarium. Properly acclimating livestock helps to equalize the different water temperatures and can significantly reduce the stress on your new animal(s).

    Salinity

    It is also likely the water inside the bag/package your new livestock arrived in has a different salinity than the water inside your aquarium. Beware: sudden changes in salinity can shock fish and inverts and may leave them more susceptible to infection and/or disease.

    Float Method

    This method is most useful for most fish and invertebrates:
    1. Switch off the quarantine tank or aquarium lighting and dim the lights in room.
    2. Open the box and inspect the bags to ensure that all animals have arrived in good health and that all the bags have arrived intact.
    3. Float the bags in aquarium for 20-25 minutes to allow the temperature to equilibrate. DO NOT open bag at this time.
    4. Open the bags and roll down the edges 1-2 inches to make the bag stable, and to keep it afloat in the tank. You may also use a clip or a clothespin to anchor the bag firmly against the edge of the aquarium.
    5. Add a quarter cup of aquarium water to the shipping bag. Make sure to keep water from the bag from spilling over into the aquarium. If you need to remove some of the water from the bag to aid in keeping the bag afloat you may do so. Do not dump the water from the bag into the tank; dump it into a bucket or down the drain.
    6. Repeat Step 5 every 3-7 minutes until the bag is full of water. This should take about 30-45 minutes.
    7. Discard half of the water and continue to repeat step 5 until the bag is full once again. This should take another 30-45 minutes.
    8. Compare the salinity of the water in the bag to the water in the tank. If they are the same proceed to step 9. If they are different repeat step 5 until the salt levels are the same.
    9. Remove the animal from bag and place them into the aquarium or quarantine tank (preferable). For hardy fish and motile invertebrates, use a soft net to do so. For tangs, or other sensitive animals, capture them with a cup, pouring as little of the bag’s water into your tank as possible. For sessile invertebrates, such as corals or anemones, you may remove them by hand. Be sure to use clean, sterile, and powder-free gloves.
    10. Discard the remaining water.
    Drip Method
    This is the most desirable method to acclimate sensitive animals to the aquarium but can be used for any fish, coral or invertebrate (many people use this method for acclimating all their livestock). It should be noted that unless you are sure that the water contained in each bag is from the same source you will want a separate holding container for each bag (you can run multiple drip lines to each container). Also if you are going to be using this method, please be present during the entire procedure to prevent spillage.
    1. Follow steps 1-3 from the Float Method above.
    2. Remove the bags from the water and open them. Gently pour the specimen into a holding container. Sterile plastic tubs or buckets work well in this application.
    3. Use airline tubing or the Pisces Pro Acclimator to set up a siphon from your main tank to the holding container . If you are using airline tubing you can tie a knot in the line to control the flow (see step 5 for flow rate).
    1. Start siphon by sucking on the free end of the airline tubing, or by forcing water into the tube using any existing pump or powerhead in the aquarium.
    2. Adjust flow to 1-3 drips/second. This will depend on water volume. You want the water volume to double in about 45-60 minutes.
    3. Once the volume has doubled, discard half of the container’s water, and resume dripping for another 45-60 minutes or until the water volume doubles once again.
    4. Compare the salt levels in the container to the tank and if they are the same go on to step 8. If they are different go back and repeat step 6 until the salt levels are the same. You may also repeat step 6 for extra sensitive animals.
    5. Remove the animal from container and place them into the aquarium or quarantine tank. For motile invertebrates, use a net to do so. For sessile invertebrates, such as corals or anemones, you may remove them by hand. Be sure to use clean, sterile, and powder-free gloves.
    6. Discard the remaining water.
    Other Tips
    1. Take your time and be patient.
    2. Keep plenty of new salt water on hand to maintain your water level in your quarantine tank or aquarium during the acclimation process.
    3. If any animal is without water, introduce it immediately into the quarantine tank or aquarium. Many invertebrates are inter-tidal, and are accustomed to periods of little to no water.
    4. If an animal arrives and looks dead, acclimate it anyway. Many animals will make a quick and dramatic recovery when properly acclimated.
     
    Last edited by a moderator: 31 Jan 2017
    Antionette, Monde, colbar and 3 others like this.
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  3. lanzo

    lanzo Sponsor

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    cool:)
     
  4. jacquesb

    jacquesb Retired Moderator

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    Thanks, Mekaeel! This article is a necessity!
     
  5. OP
    Mekaeel

    Mekaeel Moderator

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    cool stuff Jacques,found some free time to post something informative for a change lol
     
  6. shan

    shan

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    this is good info - just to add that the drip method works fatastically with clams
     
  7. viper357

    viper357 Admin MASA Contributor

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    Nice thread, thanks Mekaeel.

    Thread sticky'd.
     
  8. Warr7207

    Warr7207

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    Great article,you need to "Wiki" it
     
  9. OP
    Mekaeel

    Mekaeel Moderator

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    Thanks Dean,Warr! let the mods and admin do whatever they wish with the article,if its ok for the Wiki,then put it through :)
     
  10. Brian Grobler

    Brian Grobler

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    I have to maybe make everyone aware, one can purchase a human drip from many pharmacies, they dont cost much R10.00 at the most. I use them and drip from the main tank and every 15 min I remove half of the water that is in the container. (I use a small bucket) I drip them for one to one and a half hours. I did this with my Koi and tropical fish and had a huge result in a no death syndrome. I always discarded the water after the dripping and only transferrred the fish. The drip makes it so much easier to view and one can adjust it accordingly.
     
  11. jacquesb

    jacquesb Retired Moderator

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    Thanks Brian - this definitely seems to work. The only problem that I have with the "drip method", is that the water in the bag will QUICKLY run out of oxygen for the fish.... The fish usually use up all the available oxygen, if this process is not timed correctly!
     
  12. Kanga

    Kanga Retired Moderator

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    Indeed damn handy article, see if you can email Keith MacNeil and if he would let us use it in the wiki.
     
  13. keyaam

    keyaam

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    I use the drip method with a battery operated air pump to pump air and acclimatise for about 1-2 hours
     
  14. Andreas

    Andreas

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    So what alternative do you use Jacques?Do you add a air pump to the container?
     
  15. jacquesb

    jacquesb Retired Moderator

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    Hi Andreas - I use my trusty turkey baster - I spurt 22ml's (that's the amount of water that the turkey baster takes) into the bag, which of course also causes a miriad of tiny bubbles to go into the water, inside the bag). In such a way, I put water in - and supplies air into the water in the bag)....
    I repeat this every 4 to 6 minutes.... I continue for about 2 hours.... in which time I remove a lot of water from the bag as well.....
     
  16. Mouse

    Mouse

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    Thanks guys, this is brilliant! And now I have another use for my turkey baster :biggrin:
     
  17. Andreas

    Andreas

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    Thanks Jacques.Dont you put more stress on the fish if you spurt it in the bag?I noticed more reaction from my fish when let it fall drop by drop into the bucket.
     
  18. jacquesb

    jacquesb Retired Moderator

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    No Andreas - I cannot remember ever seeing that the fish "are MORE stressed out". At this stage, when you try and do the acclimatization, the fish are already most likely at their highest point of stress levels.....
    Therefor the increased breathing - and also - therefor the reason that they use up ALL air/oxygen in the water, quicker than normal! ;)
     
  19. Andreas

    Andreas

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    Cool thanks Jacques:)
     
  20. Brian Grobler

    Brian Grobler

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    Andreas, as you are dripping from your main tanks water there is enough oxygen entering the bag, I throw the water with the fish into a short wide bucket before I drip and not into the plastic. But if you found it works better wih a pump thats kewl.
     
  21. Andreas

    Andreas

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    Yip I drip from the main tank:)
     
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