Marine organisms under fluorescent excitation

Discussion in 'Photography' started by LikesFish, 18 Jan 2013.

  1. LikesFish

    LikesFish

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    Below are some marine crustaceans and other organisms, all under various fluorescence excitations.

    8390467845_c1f15df809_b.jpg
    20130115-DSLR_IMG_0150.jpg by pwnell, on Flickr
    Flatworm, 10x, FLUO-C4

    8390468023_30c27ebbf4_b.jpg
    20130116-DSLR_IMG_0069.jpg by pwnell, on Flickr
    Amphipod, 4x, FLUO-C4

    8390468361_e63c639d17_b.jpg
    20130116-DSLR_IMG_0109.jpg by pwnell, on Flickr
    Copepod, 20x, FLUO-C3, HF C

    8391551556_1a17667f8a_b.jpg
    20130116-DSLR_IMG_0216.jpg by pwnell, on Flickr
    Isopod, 10x, FLUO-C4

    8390468677_a77a380990_b.jpg
    20130116-DSLR_IMG_0232.jpg by pwnell, on Flickr
    Copepod, 20x, FLUO-C4, HF C

    8390469019_5f9f03af29_b.jpg
    20130116-DSLR_IMG_0397.jpg by pwnell, on Flickr
    Copepod, 40x, FLUO-C4

    8391552178_0318d95133_b.jpg
    20130116-DSLR_IMG_0505.jpg by pwnell, on Flickr
    Diatom, 40x, FLUO-C4
     
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  3. RocketRooster

    RocketRooster

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    Gorgeous.
     
  4. Damen

    Damen

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    quality pics.
     
  5. Nemos Janitor

    Nemos Janitor

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    Waldo, what make and filters used?
     
  6. OP
    LikesFish

    LikesFish

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    Semrock.
     
  7. Nemos Janitor

    Nemos Janitor

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    Waldo, please can you explain the process and equipment required.

    LED and fluorescence "POP" is a flavour of the popular aspirations aquariust. LED is doing it for the naked eye. The camera can do it with help in other ways.

    Please share your experiences.
     
  8. OP
    LikesFish

    LikesFish

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    Well you can use various different microscopes but you will need at least:

    1) Microscope with trinoc head for camera
    2) Some camera mounted to the trinoc head
    3) The microscope must support fluorescence
    4) Fluorescence cube to create correct excitation and barrier frequencies
    5) Fluorescence light source - these are quite pricey - typically MH or Xenon Arc or Mercury Vapour.

    Process? Lots of patience.
     
  9. Nemos Janitor

    Nemos Janitor

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    Is this tech only available to microscopes? Or, it seems to be available to diving and possibly aquarists photography.

    Is it only the filter that is required or is there a special flash/strobe/light that is necessary?
     
  10. OP
    LikesFish

    LikesFish

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    Nonono....

    The photos in this thread are all taken with a research grade microscope. The photos I have taken in other threads were taken with the nightsea barrier filter and a normal DSLR.
     
  11. OP
    LikesFish

    LikesFish

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    PS: The first image represents 2.2mm from left to right. The last image represents approximately 0.3mm from left to right, these cannot be taken with a normal camera.
     
  12. Nemos Janitor

    Nemos Janitor

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    OK Sorry to confuse. I understand.

    Can we continue on the basis of fluoresce from a entry level DSLR point of view . Maybe a new thread?

    Thanks Waldo :thumbup:
     

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