culturing anerobic bacteria

Discussion in 'Biological/Natural Filtration and Deep Sand Beds' started by lanzo, 25 Mar 2009.

  1. lanzo

    lanzo Sponsor

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    hi guys

    i'm doing a bit of a experiment....i got the idea from lappiesreef

    this is wat i needed.....
    #1 i got my girlfriend to get me a agar-plate from the Labs:yeahdude:(this is to grow the bacteria on)
    #2 i got a long tin that can seal very good(no air must come in the tin)
    #3 a tiny candle
    #4 i got a "monster" from the bottom of my dsb using the refractometer's "piepet"


    so this is how did it:

    i took the "monster" and striped it on the Agar...i closed the agar with its plastic cap....i then placed the agar plate in the tin....and lit the candle inside the tin...and then closed the tin


    so hopefully by tomorrow 4pm i'll have a big enough colony to seed my one tank


    Reason way i'm doing this is to see if i can boost my cycle period.:biggrin:
     
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  3. seank

    seank

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    huh, afrikaans on this one please
     
  4. lanzo

    lanzo Thread Starter Sponsor

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    where must i start in afr?:biggrin:
     
  5. Neil H

    Neil H Moderator MASA Contributor

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    lanzo, if that works ....... flippen top marks boet
     
  6. crispin

    crispin

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    i dont thinm any language will make sence of that post sean :)

    lanzo are u saying u want to grow bacteria from the lower areas of a DSB to seed a tank with?

    In order to shorten the time it takes for those bacteria to establish themselves?
     
  7. lanzo

    lanzo Thread Starter Sponsor

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    i dont thinm any language will make sence of that post sean :)

    lanzo are u saying u want to grow bacteria from the lower areas of a DSB to seed a tank with?

    yes the anerobic bacteria that lives in the bottom layers of the dsb;)

    In order to shorten the time it takes for those bacteria to establish themselves?

    yes... it normally takes a couple of weeks to get the anerobic bacteria started up(i know i waited 2-3 months for my anerobic bacteria to eat up all my no3)...so my idea is to put a big culture brewed up and ready for action in my dsb...to boost the whole prosses of waiting
     
  8. lIghty

    lIghty

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    interesting, but how you going to seed the new tank, won't they die when you open the agar-plate dish to the air or water due to the O2?
     
  9. lanzo

    lanzo Thread Starter Sponsor

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    some will die....this is what i want to test.....i want to take it out directly and put it in my dsb/ add it to the water colom

    hopefully it will work...ill add some pics for you guys tomorrow
     
  10. lIghty

    lIghty

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    is there not a way of just removing the oxygen from the DSB for a while so the bacteria can take off 1st before connecting to the system?
     
  11. seank

    seank

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    ???????


    ???????

    ????????? Candles are used to make light and they need oxygen to burn:whistling:

    ???????? Sounds like a hairy crab or Mantis:lol:
     
  12. lIghty

    lIghty

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    What about taking the agar-plate and burrying it under the sand and breaking it using a metal rod or stick:whistling:
     
  13. lanzo

    lanzo Thread Starter Sponsor

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    so what i can do is...switch of the return pumps and add co2 to the dsb chamber?
     
  14. lIghty

    lIghty

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    Candle will burn untill all oxygen is removed, therefore making it a oxygen free volume
     
  15. lIghty

    lIghty

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    Might work, thats what i had in mind:whistling:
     
  16. lanzo

    lanzo Thread Starter Sponsor

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    If one close the candle in the tin it will use up all the 02 in the tin....thus leaving u with no o2

    "NAT-SKEI MY MAAT":biggrin:

    agar is a thing used to grow all kinds of bacteria on...it looks like jelly and smells like ****:biggrin:

    i dont want any other bacteria growing on the agar...this is why the tin should seel very very good...otherwise ill get 1-50 diff kinds of bacteria
     
  17. lanzo

    lanzo Thread Starter Sponsor

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    wont the agar plate contaminate the water?

    no to find a co2 bottle and a regulator:p
     
  18. lIghty

    lIghty

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    just empty as much water out and fill with Soda Water? but wait, that has no salt..... don't you have a Soda machine?
     
  19. lanzo

    lanzo Thread Starter Sponsor

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    soda machine...no but i can try and get one.

    how many pumps do i give in that camber? and for how long should i keep it like that?(ill test with ph pen until ph is 6)

    dude...your are a genius man....this will drop the ph and no o2....ideal for the anaerobic bacteria.....lIghty the legend man:yeahdude:
     
  20. lIghty

    lIghty

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    Not sure how many pumps, trial and error :whistling: just thinking out the box ;)

    Now go hit my Reputation button LOL :lol:

    KEEP US POSTED!
     
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  21. lanzo

    lanzo Thread Starter Sponsor

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    lol...will add pics tomorrow
     
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